Callum Lynch (Michael Fassbender) soars before the execution is imminent and discovers that he has been selected by Sophia (Marion Cotillard) to participate in a program that frees mankind from the impulse to violence. After being tied to the virtual reality machine Animus, which allows users to experience the memories of their ancestors, Callum Lynch realized that he was a descendant of Aguila, an assassin who lived during the Spanish Inquisition. They were looking for an apple of Eden that could control free will. Sophia, under pressure from her father Alan (Jeremy Irons), reluctantly manipulates Callum Lynch to go back in time to find the whereabouts of the Eden Apple in the modern world, threatening his physical and mental health. But after fellow killer Musa (Michael Williams) hints at Callum Lynch, reminding him that Allen may have impure motives, Callum Lynch begins to rethink his actions and motives, and the fate of human free will also becomes uncertain...